Fluoreye for DNA quantitation


Wondering if anyone has any experience, or data with using the Fluoreye for quantitating DNA yet? E.g. Qubit reagents. I’m not expecting it to be able to match a plate reader for performance, but I’m very interested to hear thoughts on it! :slight_smile:

1 Like

Isn’t this product brand new? Not sure if there is enough in field units for someone to chime in on.


Yes, the product is new and we’re showcasing it at SLAS. Hopefully, in the near future, we will host a webinar on Fluoreye. I will have to look into what beta testing was done by my colleagues in Europe. Stateside, @NickHealy_Hamilton got a chance to set it up and assess it on the VANTAGE platform in preparation for training the internal teams. He was quite impressed by the device and its readiness for product launch!

For now though, please check out our website which has a video and flyer:


Alright, Can you put 5 on the same deck at the same time and measure 5 different plates concurrently, as long as they are on the same carrier?


For the initial launch, it will support 1 channel/device at a time. But we are eager for more feedback and ideas to make further improvements and multiple devices at a time has certainly come up! Please let me know the application and throughput needs so we understand your use case more!

1 Like

Yes… we use the qubit reagent that you can buy of the shelf and use it in our own qc for volume verification for the Tecan Fluent.

I have more info if you need, but send DM if needed

1 Like

We have created a script called ‘AirFCA QC test’ on our Tecan Fluent that we perform monthly to make sure the AirFCA is pipetting within our specified specification for Accuracy and CV for each AirFCA pipetting channel.
A deviation of <5.0 % is seen as a PASS, between 5.0% and 10.0% is seen as a WARNING and >10.0% is seen as a FAIL. If we have a fail then at least we have something to show for when we contact the vendor (never happened to this point).

We use the Qubit Flex (for strips of 8) for measurement.
Reagents = Qubit™ Flex System Verification Assay Kit - Catalog number: Q33254

Pipette 200µL blank buffer as standard 1
Pipette 200µL of Red or Green dye as Standard 2

Pipette a ratio for volume verification (we use 6 strips so 6 repeats for each channel):
X µL of blank buffer
200 - X µL of Red or Green dye

Measure standards QC-strips on the Qubit Flex and select the correct assay
2 Standards GREEN = dsDNA BR assay
2 Standards RED = RNA BR assay
3 Standards = Protein assay (possible, but not done frequently in our lab)

Mix all Qubit strips properly and spin down
Make sure the orientation of all strips is the same as during pipetting
Measure samples and export data
Used the exported measurement data to calculate the volumes for each pipetting action

In the attached picture you can see that the template we made gives all the required information.
Additional tabs for the raw Qubit data, volume calculations, interpolation plots and Report.