Selective bilayer liquid aspiration

I asked in a previous thread but decided to make a new one since I’m still having some difficulties. My goal is to have the capability of selectively removing/sampling from a layer of liquid from a mix of immiscible liquids. (Water/Dodecane or CHexane/Water are examples of ones I’m interested in). I already attempted this using PIP_PhaseAspiration from the STAR Tools library after getting the TADM curves and drawing a lower tolerance band threshold. However, when I went to test it, it still ended up sampling both liquids. Any ideas/suggestions would be super helpful

Hi @jdhawkins15 ,

Could you share a screen shot of the TADM measurements when you pipetted both top and bottom layer? Did you make sure that TADM was set to Monitoring mode prior to your test?