Hi Folks!
First time posting here, so feel free to ask for more details/direct me in the right direction for this post.
We recently ran a method that lost fidelity with a channel, and then causing an inability to initialize the robot and run any method types at all. The only thing we could find is the attached issue in the log file, making for a perplexing issue. The issue has since been resolved through restarting the machine, but interrupted a key method that resulted in a less than desirable outcome (spillage of material, etc.)
Does anybody have any ideas as to what could cause this?
Thanks!
K
Hello @Kastronaut,
The snippet indicates that something occurred prior to the lines provided that resulted in a need to abort or re-init the instrument. Could you please provide the full trace file? Additionally, when was this instrument last serviced by a Hamilton Service Engineer?
~William
3 Likes
Hi William!
Thanks for being so prompt, I’ve attached the trace file here. The system has not been serviced in quite some time.
Apologies for the lengthy post:
2022-11-11 13:02:34> SYSTEM : Analyze method - start; Method file C:\Program Files (x86)\HAMILTON\Methods\Vlad\Purification v1.1.0_05.12.22.hsl
2022-11-11 13:02:41> Microlab® STAR : Communication - progress; Connection to instrument is created.
2022-11-11 13:02:42> SYSTEM : Analyze method - complete;
2022-11-11 13:02:43> SYSTEM : Start method - start;
2022-11-11 13:02:43> SYSTEM : Start method - progress; User name: SVC-MOLBIO
2022-11-11 13:02:43> SYSTEM : Start method - progress; Phoenix software version: 4.5.0.7977
2022-11-11 13:02:44> SYSTEM : Start method - progress; Database version: Standard
2022-11-11 13:02:44> SYSTEM : Start method - progress; Sample tracking: On
2022-11-11 13:02:44> SYSTEM : Start method - progress; Vector Database: On
2022-11-11 13:02:44> SYSTEM : Start method - progress; Vector Database connection: Database ‘HamiltonVectorDB’ on Microsoft SQL Server ‘LOCALHOST\HAMILTON’ with user ‘Hamilton’
2022-11-11 13:02:45> Microlab® STAR : Start method command - start;
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Microlab® STAR software version: 4.5.0.5217
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Serial number of Instrument: F596
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of main module: 7.5S 14 2017_09_26 (GRU C0 PLE)
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of 1000µl channels: 3.9S f 2016-06-02, 3.9S f 2016-06-02, 3.9S f 2016-06-02, 3.9S f 2016-06-02, 3.9S f 2016-06-02, 3.9S f 2016-06-02, 3.9S f 2016-06-02, 3.9S f 2016-06-02
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of 5ml channels: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of labware handling channels: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of X drive: 1.4S 2012-04-25
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of auto load: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of iSWAP: 4.1S 2011-12-19
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of washer 1: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of washer 2: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of temperature-controlled carrier 1: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of temperature-controlled carrier 2: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of CO-RE 96 Head: 5.0S e 2017-03-16 (H0 XE167)
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of pump station 1: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of pump station 2: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of pump station 3: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of nano dispenser: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of nano dispenser pressure unit: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of CO-RE 384 Head: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of tube gripper: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of camera channel: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of gel card gripper: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of punch card gripper: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of puncher module: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 1 module 1: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 1 module 2: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 2 module 1: not installed
2022-11-11 13:02:45> Microlab® STAR : Start method command - progress; Firmware version of twister decapper station 2 module 2: not installed
2022-11-11 13:02:48> Microlab® STAR : Start method command - complete;
2022-11-11 13:02:48> SYSTEM : Start method - complete;
2022-11-11 13:02:48> SYSTEM : Execute method - start; Method file C:\Program Files (x86)\HAMILTON\Methods\Vlad\Purification v1.1.0_05.12.22.hsl
2022-11-11 13:02:49> Microlab® STAR : Initialize (Single Step) - start;
2022-11-11 13:02:49> Microlab® STAR : Clean up instrument - progress; Initialize channel Z drive
2022-11-11 13:02:51> Microlab® STAR : Clean up instrument - progress; Move up 1000ul channels
2022-11-11 13:02:51> Microlab® STAR : Clean up instrument - progress; Move up CO-RE 96 Head
2022-11-11 13:02:53> Microlab® STAR : - progress; User action: Message box ‘Clean up instrument’ message ‘Please check the traverse height! Is there any indication for a collision when moving horizontally?’ was closed with button ‘No’.
2022-11-11 13:03:53> Microlab® STAR : Initialize (Single Step) - progress; Error handling waiting for manual recovery!
2022-11-11 13:07:07> Microlab® STAR : Initialize (Single Step) - error; > channel 8: Waste, , E:P8:02/10:Initialize
2022-11-11 13:07:07> Microlab® STAR : Initialize (Single Step) - progress; Error manually recovered by user.
2022-11-11 13:07:20> Microlab® STAR : - progress; User action: Message box ‘Abort command’ message ‘Are you sure you want to abort the run?’ was closed with button ‘Yes’.
2022-11-11 13:07:20> Microlab® STAR : Initialize (Single Step) - error; > channel 8: Waste, , E:P8:02/10:Abort
2022-11-11 13:07:20> Microlab® STAR : Main - error; An error occurred while running Vector. The error description is: Step aborted. The method should be restarted. ([P8: Main, Hardware error.]) (0x28 - 0x1 - 0x804)
2022-11-11 13:07:20> Microlab® STAR : Initialize (Single Step) - progress; Error manually recovered by user.
2022-11-11 13:07:20> Microlab® STAR : Initialize (Single Step) - complete with error; An error occurred while running Vector. The error description is: Step aborted. The method should be restarted. ([P8: Main, Hardware error.]) (0x28 - 0x1 - 0x804) , > channel 1: Waste, > channel 2: Waste, > channel 3: Waste, > channel 4: Waste, > channel 5: Waste, > channel 6: Waste, > channel 7: Waste,
2022-11-11 13:07:20> SYSTEM : Method has been aborted by the user - complete;
2022-11-11 13:07:20> SYSTEM : Abort method - start;
2022-11-11 13:07:20> Microlab® STAR : Abort requested command - start;
2022-11-11 13:07:20> Microlab® STAR : Abort requested command - complete;
2022-11-11 13:07:20> Microlab® STAR : Start Method Abort Handler command - start;
2022-11-11 13:07:22> Microlab® STAR : Start Method Abort Handler command - complete;
2022-11-11 13:07:22> DEBUG : _Method::Util2::Debug::TraceArray - progress; Step names array trace:
2022-11-11 13:07:22> DEBUG : _Method::Util2::Debug::TraceArray - progress; Empty array(s).
2022-11-11 13:07:22> DEBUG : _Method::Util2::Debug::TraceArray - progress; Step times (s) array trace:
2022-11-11 13:07:22> DEBUG : _Method::Util2::Debug::TraceArray - progress; Empty array(s).
2022-11-11 13:07:22> DEBUG : _Method::Util2::Debug::TraceArray - progress; Step times (min) array trace:
2022-11-11 13:07:22> DEBUG : _Method::Util2::Debug::TraceArray - progress; Empty array(s).
2022-11-11 13:07:22> Microlab® STAR : Abort command - start;
2022-11-11 13:07:22> Microlab® STAR : Abort command - complete;
2022-11-11 13:07:22> SYSTEM : Abort method - complete;
2022-11-11 13:07:22> SYSTEM : Execute method - complete;
2022-11-11 13:07:22> SYSTEM : End method - start;
2022-11-11 13:07:22> Microlab® STAR : End method command - start;
2022-11-11 13:07:24> Microlab® STAR : Clean up instrument - progress; Move up 1000ul channels
2022-11-11 13:07:28> Microlab® STAR : Clean up instrument - progress; Move up CO-RE 96 Head
2022-11-11 13:07:33> Microlab® STAR : Internal command - error; An error occurred while running Vector. The error description is: Hardware error. Firmware component Main module (0x29 - 0x1 - 0xa)
2022-11-11 13:07:33> Microlab® STAR : End method command - complete;
2022-11-11 13:07:33> SYSTEM : End method - complete;
2022-11-11 13:07:35> SYSTEM : File checksum - written; $$author=SVC-MOLBIO$$valid=0$$time=2022-11-11 13:07$$checksum=dfa0c489$$length=139$$
K
It looks like you are getting a hardware error on channel 8 during the initialize step. I see that a service work order was created for this system on 11/11 to schedule a repair. I believe that would be prudent, but if you continue to have issues, please let us know.
