Im using a Tecan Freedom Evo, its kind of a older version. I have a GWl file that looks like this:
A;DNA Extraction Plate;;DNA Extraction Plate;1;;23.08;;;
D;Normalized Plate;;qPCR A tailing 0.2mL;1;;23.08;;;
A;DNA Extraction Plate;;DNA Extraction Plate;2;;33.15;;;
D;Normalized Plate;;qPCR A tailing 0.2mL;2;;33.15;;;
The last value on the “A;” line is how much volume to aspirate (for example, the first line is 23.08uL and the second line is 33.15uL. The range of volumes are 5uL-75uL.
I want the Tecan to use 200uL tips when pipetting anything above 30uL and 50uL tips when pipetting anything below 30uL. I am not sure how to do this. The script in the Tecan software just prompts the user to import the GWL file and it does the rest but it inly uses 200uL tips. I want to make an if statement that uses the volume as a variable and if it is under 30uL use 50uL tips, otherwise use 200uL tips.
I would appreciate any advice anyone has to do this. Let me know if you need more information, like how my Script looks like in Tecan workflow UI.