Checking Dispensing Volume

Hello comrades!

Colud you please share your experiance at checking dispernsing volume? How do you do it? What devices do you use or methods? At my job we have Echo that we can check volume beatwen 2-60 uL, and also VolumeCheck for larger volumes, but I’m just wondering about other options.

Thanks!

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I recommend checking out the ISO 23783 documents for guidance on volume checks. The ISO 23783 guidelines consist of three parts that are specific to automated liquid handling systems (ALHS):

The ISO 23783 Part 2 document is most pertinent to your questions as it applies to all ALHS accounting for a wide variety of technologies and tests that can be performed to assess pipetting performance. There are a lot of approaches, each has its pros and cons, sensitivities, volume ranges, etc.

At Hamilton, we employ several of these measurement methods depending on the pipetting technology and needs.

  • Liquid Verification Kit (LVK) - gravimetric verification and liquid class development tool
  • Field Verification Kit 2 (FVK2) - hybrid photometric/gravimetric that is used as an internal tool for testing all pipetting devices as part of the IQ/OQ testing at the time of installation and PMs
  • VeriPlate - optical image analysis of liquid-filled capillaries for routine tests

The VeriPlate is something new we showcased at SLAS that will launch soon and is part of our larger Hamilton Pipetting Excellence initiative. This link will get you access to the whitepaper I recently co-authored with my colleagues. Also, see here for the Liquid Handling Reference guide that includes a section on measuring liquid transfers.

Liquid handling in general and verification of said liquid handling as it pertains to automation are topics that have been of great interest and even passion of mine throughout my career. In addition to the tools Hamilton provides, we also have active collaborations with vendors such as Artel so our customers have many options based on their specific needs. I am very eager to hear everyone’s thoughts in this thread!

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Hi Eric,

Thanks for this wealth of information! When I go to download the white paper, however, I get a “404 not found” error. Is this on my end or are others experiencing this as well?

I see the issue - it was working before! I let the marketing and website team know and will update you when it’s been corrected.

UPDATE: It’s been fixed!

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Thanks, Eric!

Volume verification has been a critical issue in our operations for decades. It’s great to see more solutions enter the marketplace to help us with quality of results.

In my view, volume verification is divided into two camps.

Reference methods deliver a static reading of liquid handler performance at a given point in time. You validate your liquid handler periodically and then proceed with your assays hoping that the performance level you measured previously remains fairly consistent. Examples of reference methods are the various dye-based routines and capillary measurements.

Real-time, in-line solutions measure your actual samples in the process microplate with your system fluids. These can be run on every plate in an assay and data recorded for each well in every plate, at every addition/aspiration if you desire. This results in actionable in-process data which can then be utilized to facilitate true ratiometric dilutions, flag liquid handler/device errors, audit incoming levels, and so on. Examples of in-line solutions are acoustic measurement, ultrasonic, conductive/capacitive pipet tip, pressure-based, and optical.

I’m working with Meniscense, https://www.meniscense.com/, who launched their VolumeSense production device at SLAS 2024. The VolumeSense uses optical interferometry to provide a high-resolution and non-contact means for volume measurement, in-line with your method.

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